International Conference on Biochemistry October 10-12, 2016 Kuala Lumpur, Malaysia

Biography:

Venkataramanan Swaminathan currently working as a senior Lecturer in Management and Science University, shah alam, Malaysia, Basically he has Double Master’s Degree first master in Biochemistry and Second master’s in Bioinformatics. He has app 12 years’ experience in Teaching/Academic Field. He had participated many International Conference like Pharmacy, Forensic and Computer Science. He has international publication in National and International Journal’s. He had supervised various students Projects both bachelor and master’s too. He had been involved in research projects like Research Seed Grant, FRGS (Malaysian Government Grant). His current research area is Hypothetical protein in Gastric Cancer.

Abstract:

  Momordica charantia    is commonly known as bitter gourd is economically important medicinal plant. Bitter gourd comes in a variety of shapes and sizes. The substances that contain in bitter gourd are   quinine  ,  morodicine , and monorcharins. Since bitt er gourd has the monorcharins as its component therefore high consumption of bitter gourd may cause liver toxicity. Liver is a main organ in regulating homeo stasis in the body. It involves all biochemical pathways related to growth, fight against disease, nutrient supply, energy provision and reproduction. In order to have and maintain a healthy liver is very crucial. The general term used for liver damage will be hepatotoxicity. The potential hepatotoxic effect from oral administration of aqueous extraction of Momordica charantia which was tested with albino Wistar rats . The Twenty adult male rats were divided equally into four groups with 5 rats in each .Group A kept as a control while group B , C and D were respectively fed with 500mg/kg body weight , 600mg/kg body weight and 700mg/kg of the  extract daily  for 14 days . On the 15th day blood was withdrawn and centrifuged for serum to test ALT, AST and total protein . Final result of this study shows that AST, ALT and total protein was high significantly compared to control group with P<0.05. In conclusion, the Momordica charantia aqueous extract causes hepatotoxicity at higher dosage.

Biography:

Syed Hussain Mir is assistant professor in    Clinical Biochemistry    at University of Kashmir, INDIA. Since Sept. 2012,  he is visiting scientist in the group of Prof. Carola Hunte at   Institute of Biochemistry   and  Molecular Biology , University of Freiburg, Germany. He has   obtained his PhD under the guidance of Prof. Hartmut Michel (Nobel Prize winner, 1988) at Max Planck Institute of Biophysics/ JW Goethe University of Frankfurt, Frankfurt, Germany. He standardized and established methods for t he production of recombinant antibody fragments against difficult membrane protein targets, under the thesis title of “Generation of  recombinant antibodies  against membrane proteins by phage display”. As a result of the quality of this work, on august 2007, his PhD thesis was awarded as “ausgezeichnet” ( summa cum laude), which corresponds to the highest grades of German university doctorate-thesis award system. His research interests include generation of monoclonal antibodies against difficult membrane protein targets for the structural and biochemical characterization of latter. He employs Phage display system utilizing avian immune system.

Abstract:

Membrane proteins are challenging targets for crystallization and structure determination by X-ray crystallography. Antibody mediated crystallization has a major impact on the advancing structural and functional characterization of difficult membrane proteins1,2,3. More than 26 unique structures of  membrane protein  - antibody complexes have already been determined. An update of methods for generation of recombinant antibodies from hybridomas and their production in E. coli was recently published (Mir et al. 2015)3. The limited availability of suitable hybridoma cell lines due to low  immunogenicity  of therapeutically important human membrane proteins in mice has impeded the high-throughput application of this approach. Here we show an efficient method to obtain high affinity binders against dif ficult targets by phage display exploiting the avian immune system. The recombinant chicken antibodies were generated against Na+/H+ transporter (STNhaA) and used for its structural characterization. The strategy of avian immune phage display libraries provi des fast access to versatile tools for structural and functional studies and in general paves the way to generate versatile tools for research, diagnostics and therapeutics targeting membrane proteins and is of special interest for antigens highly conserved in mammals. 

Biography:

Hisham Al-Matubsi has completed his PhD from Victoria University-Australia and worked at different academic levels in different reputable academic organizations such as Victoria University-Victoria, Australia, Cincinnati University-Ohio, USA and now at The University of Petra-Amman, Jordan. Dr Al-Matubsi’s professional interests are in the area of reproductive  physiology  research, with a specific emphasis on ovarian hormones and changes that may be involved in the mechanism(s) underlying diabetic fetopathy. He has published more than 20 papers in reputed journals and is serving as an editorial board member of repute journal Diabetes and related Disorders.     

Abstract:

Uncontrolled    diabetes mellitus    (DM) is an etiological factor for recurrent pregnancy loss and major congenital malformations in the offspring. Antioxidant therapy has been advocated to overcome the oxidant-antioxidant disequilibrium inherent in diabetes. Our aims were to eval uate the protective effect of   lipoic acid   (LA) on fetal outcome and to  elucidate changes that may be involved in the mechanism(s) implicit   diabetic fetopathy  . Female rats were rendered  hyperglycemic  using streptozocin and then mated with normal male rat. Pregnant non-diabetic (group1 ; n=9; and group2; n= 7) or pregnant diabetic (group 3; n=10; and group 4; n=8) rats were treated daily with either LA (30 mg/kg body weight; groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15.  On day 15 of gestation, the rats were sacrificed, and the fetuses, placentas and membranes dissected out of the uterine horns. Following morphological examination, the fetuses, placentas and membranes were  homogenized , and used to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2 (PGEM) and PGF2a (PGFM) levels. Maternal liver and plasma total glutathione levels were also determined.Supplementation of diabetic rats with LA was found to s ignificantly (p<0.05) reduced resorption rates in diabetic rats and increased mean fetal weight compared to vehicle-treated diabetic (V-TD) group. Treatment of diabetic rats with LA (LA-TD) leads to a significant (p<0.05) increase in liver and plasma total glutathione, in comparison with V-TD rats.Decreased levels of PGEM and elevated levels of PGFM in the fetuses,  placentas  and membranes were characteristic of experimental diabetic gestation associated with malformation. LA treatment to diabetic mothers failed to normalize levels of PGEM to the v ehicle–treated  control rats. However, the levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p< 0.05) higher than those in malformed fetuses from V-TD rats.LA can reduce congenital malformations in the offspring of diabetic rats at day 15 of gestation. Thus, LA treatment did not completely prevent the occurrence of malformations, other factors, such as arachidonic acid deficiency and altered prostaglandin metabolism may be involved in the pathogenesis of the diabetes-induced congenital malformations.

Biography:

Dr. M. Walid Qoronfleh is currently the    Biotechnology    Development Director at QBRI. Walid has over 20 years of scientific, technology, business, and commercial experience with a wide range of operational and strategic responsibilities including strategic planning, scientific direction, R&D management and product development, business development, and marketing & sales strategy. Walid has held several senior management and exe cutive positions with increasing responsibilities at   GSK  , Sanofi-Aventis, NIH-NCI, AntexPharma,  ThermoFisher ,   NextGen Sciences (VP,  Personalized Medicine ) and SDIX (Executive Director, Life Science & Diagnos tics business, Nasdaq: SDIX).Dr. Qoronfleh is the founder of three biotechnology companies and he is a co-Founder and the Managing Director of the boutique consulting company Q3CG. He is an ad hoc reviewer for various scientific journals and a frequent speaker at international conferences. He is also editor of two journals He obtained his PhD from The University of Louisville–School of Medicine; he received his MBA from Penn State Univ ersity and acquired certification in Marketing from The University of Wisconsin–Madison.

Abstract:

It is said that “an ounce of prevention is worth a pound of cure”.  e-Health , the nexus between technology, clinical biochemistry and medicine, promises to improve people’s lives an d disease treatment. Indeed, it enables physicians in many different ways. Digital health permits better health management of patient’s conditions and it leads to h ealthy lifestyle from measuring vital signs, to diagnosing symptoms, to tracking medication use, to dieting, to exercising, etc. Digital and mobile health will not only transform point-of-care but also it will further facilitates progression towards personalized medicine to offer tailored treatment to patients. 

Biography:

I am Akansha Pant, working as ICMR Senior Research Fellow and registered as Ph.D. student, at National Institute of  Malaria Research , New Delhi, India. Currently, working on hotspot residues of falcipains and their inhibitors. I am also characterizing substrate-protease interactions of Plasmepsin V and its mutants. This work is valuab le to find alternative antimalarial drug targets.

Abstract:

 Falcipains  are among the critical enzymes req uired for parasite machinery  in Malaria. Our previous study suggested that t hey have unique pro and mature domains that interact via salt bridge and hydrophobic interactions, which are essential for their activation. Designing small  molecules that interfere at the hotspot residues of domains, would inhibit falcipains activation. Although multiple active site inhibitors exist for falcipains, specific inhibitors that halt processing without binding to active site remains unknown. Our study suggests that       azapeptide       compounds based on conformationally constrained disubstituted β- and γ-     ami         no acids    , inhibit the activation of falcipains. Among these, C-02 and C-07 hinders  the falcipains activity by binding to intact pro-FP2 rather than mature a ctive FP2 during hemoglobin hydrolysis and     fluorogenic     substrate assay. While these    compounds    did not affect the secondary structure of protein dur ing circular dichroism    spectroscopy   , Surface Plasmon Resonance result demonstrated over the range of inhibitor concentration indicated specific interaction with FP3 and equilibrium constant~80nM. Mo reover confirmation was done by MD   simulations   for ~5x130 ns confirms that compou nd-inhibitor complex provides rigidity to the pro domain to remain intact even at low pH preventing activation of the enzyme. For further authentication inhibitory concentration (IC50), of compound were examined on 3D7 strain of   Plasmodium falciparum  , parasite shows distorted  trophozoite  morphology with IC50~250nM. Further, we report a conserved histidine residue (His205) in pro domain of FP3, essential for pH sensing during auto-processing. Collectively, we provide a framework for targeting hotspot residues that can regula  te falcipains in  zymog en condition and halts i ts activation.

Biography:

Venkataramanan Swaminathan currently working as a senior Lecturer in Management and Science University, shah alam, Malaysia, Basically he has Double Master’s Degree first master in Biochemistry and Second master’s in Bioinformatics. He has app 12 years’ experience in Teaching/Academic Field. He had participated many International Conference like Pharmacy, Forensic and Computer Science. He has international publication in National and International Journal’s. He had supervised various students Projects both bachelor and master’s too. He had been involved in research projects like Research Seed Grant, FRGS (Malaysian Government Grant). His current research area is Hypothetical protein in Gastric Cancer.

Abstract:

Momordica charantia is commonly known as bitter gourd, economically important medicinal plant. Bitter gourd comes in a variety of shapes and sizes. The substances that contain in bitter gourd are quinine, morodicine and monorcharins. Since bitter gourd has the monorcharins as its component, therefore high consumption of bitter gourd may cause liver toxicity. Liver is a main organ in regulating homeostasis in the body. It involves all biochemical pathways related to growth, fight against disease, nutrient supply, energy provision and reproduction. In order to have and maintain a healthy liver it is very crucial. The general term used for liver damage will be hepatotoxicity. The potential hepatotoxic effect from oral administration of aqueous extraction of Momordica charantia which was tested with albino Wistar rats. The 20 adult male rats were divided equally into four groups with 5 rats in each. Group A kept as a control while group B, C and D were fed with 500 mg/kg body weight, 600 mg/kg body weight and 700mg/kg of the extract daily for 14 days, respectively. On the 15^th day blood was withdrawn and centrifuged for serum to test ALT, AST and total protein. Final result of this study showed that AST, ALT and total protein was high significantly compared to control group with P<0.05. In conclusion, the Momordica charantia aqueous extract causes hepatotoxicity at higher dosage.

Biography:

Yui Umekawa is a PhD candidate in   biochemistry  ,  bioenergetics , molecular biology at The United  Graduate Sch ool of Agricultural Sciences, Iwate University, Japan.

Abstract:

Temperature is one of the most important requirements for all living    organisms   . To survive in severe environments in which temperature changes continuously,  some animals have gained the ability to maintain their temperature during the   evolutionary process  , called  homoeothemy , which is performed by a complex mechanism involving ther  mal receptors throughout the body and integration in the  hypothalamus  that controls shivering and non-shivering thermogenesis. Interestingly, flowers of some plants show a similar homeothermic b ehavior by inversely controlled respiration to temperature. To clarify the thermo regulatory mechanism in thermogenic plants, we investigated the temperature response of respiration in vivo using modified Arrhenius model using homeothermic spadices of skunk cabbage (Symplocarpus renifolius). Our results clearly showed that overall    thermodynamic    activation energy exhibits a negative value in the temperature range in which respiration control occurs. Our results suggest  that respiratory control in this plant is achieved by a   pre-equilibrium chemical   reaction in response to temperature. Moreover, citrate- driven respiration analysis using isolated   mitochondria   from  thermogenic spadices further suggests that chemically  endothermic  reactions, such as NADPH production catalyzed by mitoc  hondrial isocitrate dehydrogenase, are involved in our proposed pre-equilibrium reaction. A law of chemical equilibrium known as Le Châtelier's princip le may govern the homeothermic control in  skunk cabbage.

Biography:

Debora Roselita Karo Sekali has completed her Bachelor in Medical Science from Universitas Indonesia in 2016. She is currently pursuing her Honors Degree in Research of Reproductive Physiology of Women with prolonged labor in Monash University, Australia.

Abstract:

In Indonesia, approximately 20% of couples are infertile and 40% of the cases caused by male infertility. Impaired sperm motility (asthenozoospermia) is the leading cause of inability to conceive after regular unprotected sex in one year. Reactive Oxygen Species (ROS) is associated with male reproductive health. However, biochemical analysis on infertile males are rarely done in a clinical practice and WHO laboratory manual ranges of seminal fluid characteristic can be different in various races and ethnicities. Objective of this study is to compare malondialdehyde concentration as an indicator of ROS level in seminal plasma between normozoospermia and asthenozoospermia and to find the correlation between level of malondialdehyde and sperm motility among infertile males. Case control study analyzes 15 asthenozoospermic males and 20 normozoospermic males’ seminal plasma in a fertility clinic in Jakarta. Thiobarbituric acid assay is used to measure the concentration of malondialdehyde. Mann-Whitney test shows, there is no correlation between concentration of malondialdehyde and asthenozoospermia p value=0.194. However, average of malondialdehyde concentration in normozoospermia is lower than in asthenozoospermia. The future research with the same topic should be done by choosing fertile men as the control group and by taking minimal two samples for each subject since the population variances are unequal.

Biography:

Palina Vyhouskaya is a PhD student at the Jagiellonian University, Poland. She is a member of The Scientific Students Association of Laboratory  Diagnosticians , where she gains experience and practice connected with modern research methods used in Medicine. 

Abstract:

In oral cavity conditions, cariogenic bacteria   Streptococcus mutans   are characterized by altered   metabolism   compared to cells found in physiological flora. The metabolism of Streptococcus mutans is based on   glycolysis  , which also occurs in presence of oxygen (a phenomenon known as the Warburg effect). The low concentration of oxygen (<2%), i.e., hypoxia inside the biofilm, increases an expression of genes encoding glycolytic enzymes and i nhibits the oxidative  phosphorylation . Pyruvate kinase (PK) , one of the enzymes involved in glycolysis, is considered as an enzyme conditioning the rate of the whole process since it is activated by glucose-6-phosphate, a substrate of glycolysis. Pyruvate kinase  from  S. mutans ATCC and 40 clinical strains was purified, precipitated and estimated  fluorimetrically . Here we revealed the activity, and regulation of PK in mixed bacterial biofilm species and discuss how these properties enable regulation of PK for cariogenic biofilm proliferation and caries pro gression consequently. Clinical strains were isolated from children with caries. Mixed biofilm assay was carried out according to Current Protocols in Microbiology.PK activity was higher  (1.65 mU/mg of protein) in the mixed cariogenic biofilm species compared to the single and mixed physiological biofilm types (1.15 mU/mg of protein vs. 1.33 mU/mg of protein).It was demonstrated that the pyruvate kinase activity is increased in mixed cariogenic biofilm species. Streptococcus mutans are more resistant to glycolytic enzyme inactivation occurring in mixed cariogenic biofilm species (including Streptococcus sobrinus, Lactobacillus acidophilus, and Actinomyces viscosus) compared to mixed physiological biofilm types. Inhibition  of glycolytic enzymes might be an essential step in the reduction of mixed cario genic biofilm species which could be a useful tool in caries prophylaxis.

Biography:

Naureen Fatima has completed her studies from the Aligarh Muslim University (AMU), India. She has completed her graduation in 2009 and Post-graduation in 2011. In 2013, she has joined for her PhD at the Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, under the supervision of Dr. Shagufta Moin, Department of Biochemistry and co-supervision of Professor M Owais, Interdisciplinary Biotechnology Unit, Aligarh Muslim University. She has two research publications, one in Elsevier Journal, Appetite in 2013 as a second author and another in PLOS One in 2016 as a co-author. She has received the Best Poster Award in the 3^rd Annual Meeting of Indian Academy of Biomedical Sciences and Symposium on Modern Trends in Human Diseases in December, 2013 held at the Department of Biochemistry, J.N. Medical College, AMU, India.

Abstract:

Background: Curcumin, an active component of turmeric has caught tremendous attention as a potential therapeutics for diabetes because it is a relatively safe and inexpensive drug that reduces  glycemia  and hyperlipidemia in rodent models of diabetes. 

Aim: To study the effect of in-house synthesized curcumin nanoparticles (Cur NPs) in the treatment of type 2 diabetes in experimental Wistar rat model.

Method: The Streptozotocin (STZ)-induced experimental rat model of diabetes were used to evaluate the effect of in-house synthesized curcumin nanoparticles (Cur NPs) on glycemia, body weight, glycosylated  hemoglobin  (HbA1c), oxidative stress and inflammatory immunological markers. 

Results: In this study, we developed Aloe vera leaf extract (AVLE) mediated curcumin nano-formulation for highly effective diabetes therapy. Polyphenol (AVLE) mediated bio-functional, Cur NPs showed excellent dispersibility and outstanding stability in physiological environments. The data of biochemical and inflammatory  biomarkers  revealed the ameliorative effect of Cur NPs on STZ-induced diabetic experimental Wistar rat model. Interestingly, administration of Cur NPs for four weeks was able to prevent body weight loss, reduce the levels of glucose, hemoglobin (Hb), and HbA1C in blood and improve insulin sensitivity. 

Conclusions: The data of the present study clearly showed that the therapeutic efficacy of the AVLE synthesized Cur NPs were found better than that of free form of curcumin as well as with the AVLE alone. Cur NPs were also found to be effective in ameliorating the increased levels of fasting blood glucose, urine sugar, and urine volume in STZ-induced diabetic rats. Polyphenol mediated green synthesis of Cur NPs with effective and efficacious anti-diabetic potential may open new prospects for type 2 diabetes therapy.

Biography:

Zeba Farooqui is currently pursuing PhD at the Department of Biochemistry, Aligarh Muslim University (AMU), India. She is currently working on “protective effect of Nigella sativa and thymoquinone on cisplatin induced toxicity in rat kidney”. She has published a research article in an international journal. She has presented her work in several scientific meetings and conferences.

Abstract:

Nephrotoxicity is a severe complication in patients undergoing cisplatin (CP) chemotherapy. Thymoquinone (TQ), a monoterpene isolated from volatile oil of Nigella sativa seeds has been shown to exhibit strong antioxidant properties and protective effects against oxidative damage induced by several drugs and toxicants. The present study was undertaken to investigate whether TQ can prevent the CP-induced nephrotoxic effects or not. Rats were divided into four groups: Control, CP, TQ and CP+TQ. Rats in the groups CP+TQ and TQ were administered TQ (1.5 mg/kg bwt orally), prior to and simultaneously with and without, multiple doses of CP (3 mg/kg bwt, i.p) every fourth day for 20 days, respectively. CP nephrotoxicity was evident by increased serum creatinine (Scr) and blood urea nitrogen (BUN). CP treatment caused oxidant/antioxidant imbalances as reflected by increased  lipid  peroxidation (LPO), decreased enzymatic and non-enzymatic antioxidants. Furthermore, the activities of brush border membrane (BBM) marker enzymes like alkaline phosphatase (ALP), γ-glutamyl transferase (GGTase) and leucine aminopeptidase (LAP) were significantly declined in renal cortical and medullary homogenates and in isolated BBM vesicles (BBMV) in CP treated rats. Oral TQ administration, significantly attenuated CP induced increase in Scr and BUN and decrease in BBM enzymes activities. TQ administration also precluded CP induced alterations in the enzymatic and non-enzymatic antioxidant parameters. Histopathological observations showed extensive kidney damage in  CP treated animals and remarkably reduced renal injury in CP and TQ co-treated group. The results suggested that TQ alleviates CP induced nephrotoxicity and oxidative damage by strengthening antioxidant defense mechanism in the kidney.

Biography:

Eman T Mehanna is a Lecturer of Biochemistry and Molecular Biology at the Faculty of Pharmacy, Suez Canal University, Egypt. She has 6 internationally published papers.

Abstract:

The intestinal fatty acid binding protein (FABP-2) is expressed in enterocytes and binds with saturated and unsaturated long-chain fatty acids. FABP-2 Ala54Thr polymorphism was reported to have an influence on lipid metabolism. This study aimed to assess the relation of this polymorphism with peripheral atherosclerosis combined with type 2 diabetes mellitus in an Egyptian population. The study included 100 diabetic patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by PCR-RFLP. FABP-2 level was measured by ELISA technique. FBG, fasting serum insulin, HbA1c lipid profile, BMI, systolic and diastolic blood pressure were all determined. The Thr54 allele had higher frequency among the patients group (p=0.002). The heterozygote Ala54/Thr54 and the rare Thr54/Thr54 genotypes showed significant increase in BMI and FABP-2. Carriers of Thr54/Thr54 genotype had significantly decreased HDL-C. Carriers of Thr54/Thr54 genotype had significantly higher systolic and diastolic blood pressure than carriers of both Ala54/Ala54 and Ala54/Thr54 genotypes. FABP-2 level had positive correlation with BMI, systolic and diastolic blood pressure and negatively correlated with HDL-C. The Thr54 allele of FABP-2 Ala54Thr polymorphism was associated with increased incidence of peripheral atherosclerosis combined with type-2 diabetes mellitus in the studied population.

Biography:

Kavitha has double master’s in  Bioinformatics  and bachelor in Chemistry, currently doing phd in VIT,INDIA.She has be en actively engaged in research and done few international conferences and startup publishing work in various international publications.

Abstract:

    Norfloxacin     (fluroquinolones, noroxin) admission complex from claiming bigger dosage from claiming Norfloxacin might b ring about    Cholestatic      jaundice  .   Eventually Tom's perusing hindering alternately decreased bile stream in the liver. Those instruments from claiming Cholestatic jaundice might a chan ce to be comprehensively arranged under   hepatocellular  , the place an impedance for bile structuring happens also obstructive, the place impedance will bile stream happens after it is structured. Eclipta Prostrata may be recognized an essential liver herb clinched alongside Ayurveda. Those Eclipt a Prostrata need been extensively mulled over for its  hepatoprotective  movement. The hepatoprotective movement of the watery  extricate from claiming Eclipta Prostrata might have been investigated against Norfloxacin prompted Cholestatic jaundice clinched alongside rats. Level for serum ALT, AST also aggregate  bilirubin  might have been measured on normal, control (disease/toxicity induced), Furthermore watery extricate for Eclipta Prostrata dealt with rats. During the measurement from claiming 400 mg/kg, Norfloxacin prompted Cholestatic jaundice Previously, rats Concerning illustration showed Eventually Tom's perusing statistically  huge (p<0. 05) decline over serum ALT, AST Also downright bilirubin level contrasted with control bun ch. Oral pre-treatment about rats for the watery extricate for Eclipta Prostrata during the doses from claiming 150 mg/kg, 250 mg/kg and 350 mg/kg body weight former on Norfloxacin dosing in 400 mg/kg gotten huge distinction in the exercises for serum ALT, AST also downright bilirubin level of the test bunches. These present outcomes recommend that the watery extricate for Eclipta Prostrata might have a powerful hepatoprotective impact against Norfloxacin prompted Cholestatic jaundice rats.

Biography:

Naureen Fatima has completed her studies from the Aligarh Muslim University (AMU), India. She has completed her graduation in 2009 and Post-graduation in 2011. In 2013, she has joined for her PhD at the Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, under the supervision of Dr. Shagufta Moin, Department of Biochemistry and co-supervision of Professor M Owais, Interdisciplinary Biotechnology Unit, Aligarh Muslim University. She has two research publications, one in Elsevier Journal, Appetite in 2013 as a second author and another in PLOS One in 2016 as a co-author. She has received the Best Poster Award in the 3^rd Annual Meeting of Indian Academy of Biomedical Sciences and Symposium on Modern Trends in Human Diseases in December, 2013 held at the Department of Biochemistry, J.N. Medical College, AMU, India.

Abstract:

Curcumin, an active component of turmeric has caught tremendous attention as a potential therapeutics for diabetes because it is a relatively safe and inexpensive drug that reduces glycemia and hyperlipidemia in rodent models of diabetes.To study the effect of in-house synthesized curcumin nanoparticles (Cur NPs) in the treatment of type 2 diabetes in experimental Wistar rat model.The Streptozotocin (STZ)-induced experimental rat model of diabetes were used to evaluate the effect of in-house synthesized curcumin nanoparticles (Cur NPs) on glycemia, body weight, glycosylated hemoglobin (HbA1c), oxidative stress and inflammatory immunological markers.In this study, we developed Aloe vera leaf extract (AVLE) mediated curcumin nano-formulation for highly effective diabetes therapy. Polyphenol (AVLE) mediated bio-functional, Cur NPs showed excellent dispersibility and outstanding stability in physiological environments. The data of biochemical and inflammatory biomarkers revealed the ameliorative effect of Cur NPs on STZ-induced diabetic experimental Wistar rat model. Interestingly, administration of Cur NPs for four weeks was able to prevent body weight loss, reduce the levels of glucose, hemoglobin (Hb), and HbA1C in blood and improve insulin sensitivity.The data of the present study clearly showed that the therapeutic efficacy of the AVLE synthesized Cur NPs were found better than that of free form of curcumin as well as with the AVLE alone. Cur NPs were also found to be effective in ameliorating the increased levels of fasting blood glucose, urine sugar, and urine volume in STZ-induced diabetic rats. Polyphenol mediated green synthesis of Cur NPs with effective and efficacious anti-diabetic potential may open new prospects for type 2 diabetes therapy.

Biography:

Eman T Mehanna is a Lecturer of Biochemistry and Molecular Biology at the Faculty of Pharmacy, Suez Canal University, Egypt. She has 6 internationally published papers.

Abstract:

The intestinal fatty acid binding protein (FABP-2) is expressed in enterocytes and binds with saturated and unsaturated long-chain fatty acids. FABP-2 Ala54Thr polymorphism was reported to have an influence on lipid metabolism. This study aimed to assess the relation of this polymorphism with peripheral atherosclerosis combined with type 2 diabetes mellitus in an Egyptian population. The study included 100 diabetic patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by PCR-RFLP. FABP-2 level was measured by ELISA technique. FBG, fasting serum insulin, HbA1c lipid profile, BMI, systolic and diastolic blood pressure were all determined. The Thr54 allele had higher frequency among the patients group (p=0.002). The heterozygote Ala54/Thr54 and the rare Thr54/Thr54 genotypes showed significant increase in BMI and FABP-2. Carriers of Thr54/Thr54 genotype had significantly decreased HDL-C. Carriers of Thr54/Thr54 genotype had significantly higher systolic and diastolic blood pressure than carriers of both Ala54/Ala54 and Ala54/Thr54 genotypes. FABP-2 level had positive correlation with BMI, systolic and diastolic blood pressure and negatively correlated with HDL-C. The Thr54 allele of FABP-2 Ala54Thr polymorphism was associated with increased incidence of peripheral atherosclerosis combined with type-2 diabetes mellitus in the studied population.