Hafiz Muhammad Arsalan
University of Central Punjab, Pakistan
Title: Correlation between biochemical and anti-oxidative status in Rheumatoid Arthritis patients update from Lahore, Pakistan
Biography
Biography: Hafiz Muhammad Arsalan
Abstract
Background: Rheumatoid Arthritis (RA) a prototypical inflammatory joint disease characterized by inflammation of the tissues lining the joint (synovium). Radical species with oxidative activity, Reactive Nitrogen Species (RNS) and Reactive Oxygen Species (ROS) symbolize the intermediaries and effectors of cartilage break. Meticulously, RANKL appears to be the pathogenic principle that causes bone and cartilage devastation in arthritis.
Aim: The aim of the present study was to estimate the biochemical and anti-oxidative status in patients suffering from Rheumatoid Arthritis (RA).
Methodology: It was comparative study. 5.0 ml blood sample of 60 diagnosed Rheumatoid Arthritis (RA) patients and 50 healthy individuals was taken from vein in clotted gel vials from orthopedic department of Mayo Hospital and Jinnah Hospital. Reduce Glutathione (GSH), Catalase (CAT), Superoxide Dismutase (SOD), Malondialdehyde (MDA), estimation of Nitric Oxide (NO), estimation of micronutrients (Vitamin A, Vitamin C and Vitamin E), and electrolytes
concentration by flame photometer (Na+ and K+) were estimated.
Result: The spectrophotometric reading of samples portrays that MDA level in Rheumatoid arthritis patients remarkably inflated than normal person (14.19±0.16) while in healthy individual is extremely low (3.26±0.27) and statistically significant (P=0.000<0.05). The level of GSH reduced (0.56±0.20) as compared to normal individual (6.39±0.20). The CAT level is moderately elevated in patients (6.92±0.11) than normal person (4.80±1.02). Results parade the amount of SOD that is elevated in patients (12.04±1.03) though it was high as compared to normal people (2.15±0.33). The Vitamin A level in patients (0.15±0.21) than normal person (7.21±0.04). This indicates that data is statistically significant (P꞊0.000<0.05).
Conclusion: Reactive oxygen species play vital role in bone disorder (Rheumatoid Arthritis). It has been reported that elevated Lipid peroxidation cause increased MDA level and reduced glutathione were associated with the pathogenesis of Rheumatoid Arthritis.