Biochemistry

Biography

Biography: Shireen Lamay

Abstract

It has been established that in cardiac disorders, there exists a synergistic correlation between reactive oxygen species (ROS) and TNF-alpha. We probed a systematic approach to the molecular basis of cardio protective effect of natural antioxidants like resveratrol in myocardial ischemia-reperfusion injury, which could offer a novel therapeutic opportunity against oxidative tissue damage. Peripheral blood mononuclear cells (PBMC’s) were isolated from blood of cardiac patients (n=20-30 having prior consent) by density gradient method and its monocytes (MN’s) were employed in culture studies (with and without resveratrol). The 24 hour cultures were subjected to evaluation of interleukins IL-1 and IL-6 as well as oxidative stress marker like MDA and Glutathione. The results were that the patient’s samples exhibited a remarkably suppressed glutathione levels (18.83 pg/ml; n=20; p<0.001) when compared to samples of healthy subjects (67.11 pg/ml; n=6; p<0.001); augmented levels of MDA were recorded in patient’s sample (34.23 nmol/ml; n=20; p<0.001) in comparison to healthy controls (5.12 nmol/ml; n=6; p<0.001). These observations are indicative that the defense system for antioxidants was compromised markedly in patients with myocardial infarction. A remarkable degree of amelioration in glutathione levels (59.35 pg/ml; n=20; p<0.001) coupled with an appreciable suppression in MDA levels (9.33 nmol/ml; n=20; p<0.001) were recorded in cultures treated with 20 ug/ml resveratrol. Furthermore, untreated patients cells exhibited augmented expressions of IL-1 (49.16 pg/ml; n=20; p<0.001) and IL-6 (53.11 pg/ml; n=20; p<0.001). Co-culturing with resveratrol exhibited suppression in IL-1 (12.32 pg/ml; n=20; p<0.001) and IL-6 (9.12 pg/ml; n=20; p<0.001). The IL-1 and IL-6 levels in healthy control cells (n=6) were recorded as 3.22 pg/ml and 4.09 pg/ml, respectively (p<0.001). It can be concluded that resveratrol, a potent polyphenol from grape sand also a natural antioxidant, ameliorated glutathione levels simultaneously and in turn, appreciably regulated IL-1 and IL-6 expressions in cell culture studies. Thus, in-depth studies are required to probe at the molecular level that whether resveratrol is having such potential or not.