International Conference on Biochemistry October 10-12, 2016 Kuala Lumpur, Malaysia

Biography:

Venkataramanan Swaminathan currently working as a senior Lecturer in Management and Science University, shah alam, Malaysia, Basically he has Double Master’s Degree first master in Biochemistry and Second master’s in Bioinformatics. He has app 12 years’ experience in Teaching/Academic Field. He had participated many International Conference like Pharmacy, Forensic and Computer Science. He has international publication in National and International Journal’s. He had supervised various students Projects both bachelor and master’s too. He had been involved in research projects like Research Seed Grant, FRGS (Malaysian Government Grant). His current research area is Hypothetical protein in Gastric Cancer.

Abstract:

  Momordica charantia    is commonly known as bitter gourd is economically important medicinal plant. Bitter gourd comes in a variety of shapes and sizes. The substances that contain in bitter gourd are   quinine  ,  morodicine , and monorcharins. Since bitt er gourd has the monorcharins as its component therefore high consumption of bitter gourd may cause liver toxicity. Liver is a main organ in regulating homeo stasis in the body. It involves all biochemical pathways related to growth, fight against disease, nutrient supply, energy provision and reproduction. In order to have and maintain a healthy liver is very crucial. The general term used for liver damage will be hepatotoxicity. The potential hepatotoxic effect from oral administration of aqueous extraction of Momordica charantia which was tested with albino Wistar rats . The Twenty adult male rats were divided equally into four groups with 5 rats in each .Group A kept as a control while group B , C and D were respectively fed with 500mg/kg body weight , 600mg/kg body weight and 700mg/kg of the  extract daily  for 14 days . On the 15th day blood was withdrawn and centrifuged for serum to test ALT, AST and total protein . Final result of this study shows that AST, ALT and total protein was high significantly compared to control group with P<0.05. In conclusion, the Momordica charantia aqueous extract causes hepatotoxicity at higher dosage.

Biography:

Syed Hussain Mir is assistant professor in    Clinical Biochemistry    at University of Kashmir, INDIA. Since Sept. 2012,  he is visiting scientist in the group of Prof. Carola Hunte at   Institute of Biochemistry   and  Molecular Biology , University of Freiburg, Germany. He has   obtained his PhD under the guidance of Prof. Hartmut Michel (Nobel Prize winner, 1988) at Max Planck Institute of Biophysics/ JW Goethe University of Frankfurt, Frankfurt, Germany. He standardized and established methods for t he production of recombinant antibody fragments against difficult membrane protein targets, under the thesis title of “Generation of  recombinant antibodies  against membrane proteins by phage display”. As a result of the quality of this work, on august 2007, his PhD thesis was awarded as “ausgezeichnet” ( summa cum laude), which corresponds to the highest grades of German university doctorate-thesis award system. His research interests include generation of monoclonal antibodies against difficult membrane protein targets for the structural and biochemical characterization of latter. He employs Phage display system utilizing avian immune system.

Abstract:

Membrane proteins are challenging targets for crystallization and structure determination by X-ray crystallography. Antibody mediated crystallization has a major impact on the advancing structural and functional characterization of difficult membrane proteins1,2,3. More than 26 unique structures of  membrane protein  - antibody complexes have already been determined. An update of methods for generation of recombinant antibodies from hybridomas and their production in E. coli was recently published (Mir et al. 2015)3. The limited availability of suitable hybridoma cell lines due to low  immunogenicity  of therapeutically important human membrane proteins in mice has impeded the high-throughput application of this approach. Here we show an efficient method to obtain high affinity binders against dif ficult targets by phage display exploiting the avian immune system. The recombinant chicken antibodies were generated against Na+/H+ transporter (STNhaA) and used for its structural characterization. The strategy of avian immune phage display libraries provi des fast access to versatile tools for structural and functional studies and in general paves the way to generate versatile tools for research, diagnostics and therapeutics targeting membrane proteins and is of special interest for antigens highly conserved in mammals. 

Biography:

Hisham Al-Matubsi has completed his PhD from Victoria University-Australia and worked at different academic levels in different reputable academic organizations such as Victoria University-Victoria, Australia, Cincinnati University-Ohio, USA and now at The University of Petra-Amman, Jordan. Dr Al-Matubsi’s professional interests are in the area of reproductive  physiology  research, with a specific emphasis on ovarian hormones and changes that may be involved in the mechanism(s) underlying diabetic fetopathy. He has published more than 20 papers in reputed journals and is serving as an editorial board member of repute journal Diabetes and related Disorders.     

Abstract:

Uncontrolled    diabetes mellitus    (DM) is an etiological factor for recurrent pregnancy loss and major congenital malformations in the offspring. Antioxidant therapy has been advocated to overcome the oxidant-antioxidant disequilibrium inherent in diabetes. Our aims were to eval uate the protective effect of   lipoic acid   (LA) on fetal outcome and to  elucidate changes that may be involved in the mechanism(s) implicit   diabetic fetopathy  . Female rats were rendered  hyperglycemic  using streptozocin and then mated with normal male rat. Pregnant non-diabetic (group1 ; n=9; and group2; n= 7) or pregnant diabetic (group 3; n=10; and group 4; n=8) rats were treated daily with either LA (30 mg/kg body weight; groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15.  On day 15 of gestation, the rats were sacrificed, and the fetuses, placentas and membranes dissected out of the uterine horns. Following morphological examination, the fetuses, placentas and membranes were  homogenized , and used to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2 (PGEM) and PGF2a (PGFM) levels. Maternal liver and plasma total glutathione levels were also determined.Supplementation of diabetic rats with LA was found to s ignificantly (p<0.05) reduced resorption rates in diabetic rats and increased mean fetal weight compared to vehicle-treated diabetic (V-TD) group. Treatment of diabetic rats with LA (LA-TD) leads to a significant (p<0.05) increase in liver and plasma total glutathione, in comparison with V-TD rats.Decreased levels of PGEM and elevated levels of PGFM in the fetuses,  placentas  and membranes were characteristic of experimental diabetic gestation associated with malformation. LA treatment to diabetic mothers failed to normalize levels of PGEM to the v ehicle–treated  control rats. However, the levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p< 0.05) higher than those in malformed fetuses from V-TD rats.LA can reduce congenital malformations in the offspring of diabetic rats at day 15 of gestation. Thus, LA treatment did not completely prevent the occurrence of malformations, other factors, such as arachidonic acid deficiency and altered prostaglandin metabolism may be involved in the pathogenesis of the diabetes-induced congenital malformations.

Biography:

Dr. M. Walid Qoronfleh is currently the    Biotechnology    Development Director at QBRI. Walid has over 20 years of scientific, technology, business, and commercial experience with a wide range of operational and strategic responsibilities including strategic planning, scientific direction, R&D management and product development, business development, and marketing & sales strategy. Walid has held several senior management and exe cutive positions with increasing responsibilities at   GSK  , Sanofi-Aventis, NIH-NCI, AntexPharma,  ThermoFisher ,   NextGen Sciences (VP,  Personalized Medicine ) and SDIX (Executive Director, Life Science & Diagnos tics business, Nasdaq: SDIX).Dr. Qoronfleh is the founder of three biotechnology companies and he is a co-Founder and the Managing Director of the boutique consulting company Q3CG. He is an ad hoc reviewer for various scientific journals and a frequent speaker at international conferences. He is also editor of two journals He obtained his PhD from The University of Louisville–School of Medicine; he received his MBA from Penn State Univ ersity and acquired certification in Marketing from The University of Wisconsin–Madison.

Abstract:

It is said that “an ounce of prevention is worth a pound of cure”.  e-Health , the nexus between technology, clinical biochemistry and medicine, promises to improve people’s lives an d disease treatment. Indeed, it enables physicians in many different ways. Digital health permits better health management of patient’s conditions and it leads to h ealthy lifestyle from measuring vital signs, to diagnosing symptoms, to tracking medication use, to dieting, to exercising, etc. Digital and mobile health will not only transform point-of-care but also it will further facilitates progression towards personalized medicine to offer tailored treatment to patients. 

Biography:

I am Akansha Pant, working as ICMR Senior Research Fellow and registered as Ph.D. student, at National Institute of  Malaria Research , New Delhi, India. Currently, working on hotspot residues of falcipains and their inhibitors. I am also characterizing substrate-protease interactions of Plasmepsin V and its mutants. This work is valuab le to find alternative antimalarial drug targets.

Abstract:

 Falcipains  are among the critical enzymes req uired for parasite machinery  in Malaria. Our previous study suggested that t hey have unique pro and mature domains that interact via salt bridge and hydrophobic interactions, which are essential for their activation. Designing small  molecules that interfere at the hotspot residues of domains, would inhibit falcipains activation. Although multiple active site inhibitors exist for falcipains, specific inhibitors that halt processing without binding to active site remains unknown. Our study suggests that       azapeptide       compounds based on conformationally constrained disubstituted β- and γ-     ami         no acids    , inhibit the activation of falcipains. Among these, C-02 and C-07 hinders  the falcipains activity by binding to intact pro-FP2 rather than mature a ctive FP2 during hemoglobin hydrolysis and     fluorogenic     substrate assay. While these    compounds    did not affect the secondary structure of protein dur ing circular dichroism    spectroscopy   , Surface Plasmon Resonance result demonstrated over the range of inhibitor concentration indicated specific interaction with FP3 and equilibrium constant~80nM. Mo reover confirmation was done by MD   simulations   for ~5x130 ns confirms that compou nd-inhibitor complex provides rigidity to the pro domain to remain intact even at low pH preventing activation of the enzyme. For further authentication inhibitory concentration (IC50), of compound were examined on 3D7 strain of   Plasmodium falciparum  , parasite shows distorted  trophozoite  morphology with IC50~250nM. Further, we report a conserved histidine residue (His205) in pro domain of FP3, essential for pH sensing during auto-processing. Collectively, we provide a framework for targeting hotspot residues that can regula  te falcipains in  zymog en condition and halts i ts activation.

Biography:

Venkataramanan Swaminathan currently working as a senior Lecturer in Management and Science University, shah alam, Malaysia, Basically he has Double Master’s Degree first master in Biochemistry and Second master’s in Bioinformatics. He has app 12 years’ experience in Teaching/Academic Field. He had participated many International Conference like Pharmacy, Forensic and Computer Science. He has international publication in National and International Journal’s. He had supervised various students Projects both bachelor and master’s too. He had been involved in research projects like Research Seed Grant, FRGS (Malaysian Government Grant). His current research area is Hypothetical protein in Gastric Cancer.

Abstract:

Momordica charantia is commonly known as bitter gourd, economically important medicinal plant. Bitter gourd comes in a variety of shapes and sizes. The substances that contain in bitter gourd are quinine, morodicine and monorcharins. Since bitter gourd has the monorcharins as its component, therefore high consumption of bitter gourd may cause liver toxicity. Liver is a main organ in regulating homeostasis in the body. It involves all biochemical pathways related to growth, fight against disease, nutrient supply, energy provision and reproduction. In order to have and maintain a healthy liver it is very crucial. The general term used for liver damage will be hepatotoxicity. The potential hepatotoxic effect from oral administration of aqueous extraction of Momordica charantia which was tested with albino Wistar rats. The 20 adult male rats were divided equally into four groups with 5 rats in each. Group A kept as a control while group B, C and D were fed with 500 mg/kg body weight, 600 mg/kg body weight and 700mg/kg of the extract daily for 14 days, respectively. On the 15^th day blood was withdrawn and centrifuged for serum to test ALT, AST and total protein. Final result of this study showed that AST, ALT and total protein was high significantly compared to control group with P<0.05. In conclusion, the Momordica charantia aqueous extract causes hepatotoxicity at higher dosage.

Biography:

Dr. B.K.Manjunatha Goud has completed his MD Biochemistry from Kasturba Medical College Mangalore, Manipal University. Has total 7 years of experience in teaching medical, dental, nursing and pharmacy students. (Melaka Manipal Medical College for 2 years as Assistant Professor of Biochemistry and currently working in Ras Al Khaimah medical and Health Sciences University, UAE since 5 years). Has published more than 52 publications both national and international journals and reviewer for various indexed journals. Also contributed to a book chapter (one of which in diabetes area). Main areas of research are Diabetes Mellitus, Cancer, Molecular Biology, Medical education.

Abstract:

 Type 2 diabetes  (previously called “adult onset diabetes”) is the most common form of diabetes accounting for about 90 to 95 percent of all diabetes cases. Diabetes can have a significant impact on quality of life by increasing risk for a variety of complicat ions. Diabetic nephropathy is one of the  most serious complications of diabetes and the most common cause of end stage renal disease.Presence of microalbuminuria (MA) is an early indicator of diabetic nephropathy.Microalbumin is routinely done to monitor the progression of nephropathy.The measurement of glycosylated hemoglobin (GHb) is one of the wellestablished means of monitoring glycemic control in patients with diabetes mellitus.Glycosylation is a nonen zymatic reaction between free aldehyde group of glucose and free amino groups of proteins. The biosynthesis of glycosylated hemoglobin’s (HbA1a, HbA1b, and HbA1c) occurs slowly, continuously and almost irreversibly throughout the four month life span of erythrocytes and the process is nonenzymatic.Recent reports have shown that the concentration of total glycosylated hemoglobin measured by commonly used methods may change significantly over a period of hours. This reflects the short term fluctuations in glucose concentration. It is now realized that these rapid changes depend on the synthesis or dissociation of the labile fraction of HbA1c, which is not separable from the stable form of HbA1c, by most routine methods. Physicians should be aware of the expected variation in HbA1c measurements as well as factors which can interfere with the estimation of microalbumin, So that high standards are maintained while estimating the MA levels and glycated hemoglobin.

Biography:

I joined Ph.D. at the Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh under the supervision of Dr. Shagufta Moin, Department of  Biochemistry , and co-supervision of Prof. M. Owais, Interdisciplinary Biotechnology Unit, A.M.U. The topic of my Ph.D. thesis is “Functional and Clinical Relevance of T-helper 17 cells in Diabetes Mellitus”. Th17 cells, which are a newly identified subset of T helper cells, have been found to be important in the pathogenesis of other autoim mune diseases. Its function is relatively unclear in type 1 diabetes.

Abstract:

Curcumin, an active component of turmeric has caught tremendous attention as a potential therapeutics for diabetes because it is a relatively safe and inexpensive drug that reduces  glycemia  and hyperlipidemia in rodent models of diabetes.The Streptozotocin (STZ)-induced experimental rat model of diabetes were used to evaluate the effect of in-house synthesized curcumin nanoparticles (Cur NPs) o n glycemia, body weight, glycosylated haemoglobin (HbA1c), oxidative stress and inflammatory immunological markers. In this study, we developed Aloe Vera leaf extract (AVLE) mediated curcumin nano-formulation for highly effective diabetes therapy. Polyphenol (AVLE) mediated bio-functional, Cur NPs showed excellent dispersibility and outstanding stability in physiological environments. The data of biochemical and inflammatory biomarkers revealed the ameliorative effect of Cur NPs on STZ-induced diabetic experimental wistar rat model. Interestingly, administration of Cur NPs for 4 weeks was able to prevent body weight loss, reduce the levels of glucose, hemoglobin (Hb), and HbA1C in blood and improve insulin sensitivity.The data of the present study clearly showed that the therapeutic efficacy of the AVLE synthesized Cur NPs were found better than that of free form of curcumin as well as with the AVLE alone. Cur NPs were also found to be effective in ameliorating the increased levels of fasting blood glucose, urine sugar, and urine volume in STZ-induced diabetic rats. Polyphenol mediated green synthesis of Cur NPs with effective and efficacious anti-diabetic potential may open new prospects for type 2 diabetes therapy.

Biography:

Zeba Farooqui is pursuing Ph.D. from Department of Biochemistry, A.M.U, Aligarh. She is presently working on “Protective effect of Nigella sativa and thymoquinone on cisplatin induced toxicity in rat kidney”.  She has published a research article in an international journal. She has presented her work in several scientific meetings and conferences.

Abstract:

  Nephrotoxicity   is a severe complication in patien ts undergoing  cisplatin  (CP) chemothe  rapy. Thymoquinone (TQ), a monoterpene isolated from volatile oil of  Nigella sativa seeds has been shown to exhibit strong  antioxidant  properties and protective effects aga inst oxidative damage induced by several drugs and toxicants. The present study was undertaken to investigate w hether TQ can prevent the CP-induced nephrotoxic effects. Rats were divided into four groups viz. control, CP, TQ and CP+TQ. Rats in the groups CP+TQ and TQ were administered TQ (1.5 mg/kg bwt orally), prior to and simultaneously with and without,  multiple doses of CP (3 mg/kg bwt, i.p) every fourth day for 20 days, respectively. CP nephrotoxicity was evident by increased serum creatinine (Scr) and blood urea nitrogen (BUN). CP treatment caused oxidant/antioxidant imbalances as reflected by increased lipid peroxidation (LPO), decreased enzymatic and non-enzymatic antioxidants. Furthermore, the activities of brush border membrane (BBM) marker enzymes viz.  alkaline  phosphatase (ALP), γ-glutamyl transferase (GGTase) and leucine aminopeptidase (LAP) were significantly declined in renal cortical and medullary homogenates and in isolated BBM vesicles (BBMV)  in CP treated rats. Oral TQ administration significantly attenuated CP induced increase in Scr and BUN and decrease in BBM   enzymes   activities. TQ administration also precluded CP induced alterations in the enzymatic and  non-enzymatic  antioxidant parameters. Histopathological observations showed extensive kidney damage in CP treated animals and remarkably reduce  d renal injury in CP and TQ co-treated group.  The results suggest that TQ alleviates CP induced nephrotoxicity and oxidative damage by strengthening antioxidant defense mechanism in the kidney.

Biography:

Abstract:

  Vitamin D   is known to have diverse effects on various systems in the body. There is evidence to suggest that a link exists between the serum vitamin D st atus and  tuberculosis . The pre sent study was designed to assess the alterations in serum 25  hydroxy-vitamin  D levels in newly diagnosed sputum AFB positive pulmonary tuberculosis patients and to study the association, if any, between serum vitamin D levels and different levels of sputum sm ear positivity.  Serum  25 hydroxy-vitamin D levels  were estimated in 65 sputum AFB positive pulmona ry tuberculosis pat ients and 65 age and gender-matched healthy controls. The levels of serum 25 hydroxy-vitamin D in tuberculosis patients were not statistically different from levels in healthy controls. However among patients  with pulmonary tuberculosis there was a significant negative correlation between the levels of serum 25 hydroxy-vitamin D and levels of sputum positivity. Serum vitamin D levels negatively correlates with bacterial load in patients with active pulmonary tuberculosis. 

Biography:

Abstract:

     T2DM      is a consequence of complex interactions among multiple genetic variants and environmental risk factors. This comp lex     disorder     is  also characterized by changes in various adipokines. In this study our objective was to estimate the levels of     adiponectin    , leptin and    resistin    (ALR) in Type 2 Diabetes Mellitus (T2DM) patients, besides studying the effect of various drugs on their levels.   Study participants included 400 diabetic and 300 normal patients from the Department of    Endocrinology    and Department of Biochemistry, Govt Medical College Srinagar. Subjects were categorized under various groups i.e (Group 1: Metformin t reated) , (Group 2: Glimpiride treated) and cases were also categorized as obese with T2DM (Group A),  obese   without T2DM (Group B) and T2DM only (Group C). The serum ALR levels were estimated by  ELISA  (Alere)  , and also  biochemical  parameters were evaluated before and after treatment. Adiponectin levels were found to be significantly lower in T2DM cases as compared to controls (12±5.5 vs 22.5±7.9 μg/ml), while as leptin and resistin levels were found to be significantly higher than controls (14.3±7.4 vs 7.36±3.73ng/ml) (13.4 ± 1.56 vs 7.236± 2.129 pg/ml). Taking the effect of drugs into conside ration, the effect on adiponectin and resist  in levels were found to be highly significant in Group 2 before and after treatment (11±5 vs 19.2±4.5 μg /ml) (13.6± 2.5 vs 7.3± 2.9 pg/ml),while as more effect was observed in leptin among Group 1(metformin) treated cases (27±15 ng/ml vs 15±15 ng/ml).Further the adiponectin levels were found to be significantly lower in Group B, while as leptin and resistin levels were found to be significantly higher among obese cases when compared to  T2DM  cases only. Glimpiride also shows more effect on FBG,HbA1c% levels while as metformin shows more effect on Lipid profile levels From the study, it can be concluded that ALR levels are effected by use of antidiabetic drugs among which glimpiride shows more effect on adiponectin and resistin levels while leptin getseffected more by metformin. It can also be proposed that ALR levels are not affected by diabetes only, suggesting that their alterations in T2DM may be due to obesity as we observed more ALR changes in obese cases when compared to T2DM cases a nd so there might be an important link between adiposity and Insulin resistance.

Biography:

Suresh Kumar is Senior Lecturer in bioinformatics at Management and Science University, Malaysia. He previously worked as Senior Lecturer at National University of Malaysia, Malaysia. He has obtained his PhD from the University of Vienna, Vienna, Austria. He did his post-doctoral work at Texas State University, USA. He has six years of experience in teaching and research. His research interests include structural bioinformatics, sequence analysis, Next-generation sequence data analysis.

Abstract:

Neisseria meningitidis is a parasitic  gram-negative  bacterium best known for its role in meningitis and other forms of meningococcal disease such as meningococcemia. It is important to identify possible novel drug targets and to  thrive serogroup B vaccines against the potential pathogen because one of Neisseria meningitidis’s strains, M C58, has natural transformation capacity. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Neisseria meningitidis infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. In the complete genome of Neisseria meningitidis strain MC58, there are 2158 coding genes out of which 681 encodes for hypothetical proteins (HPs). With the h elp of various bioinformatics tools, the extensive functional analysis of these HPs was performed. We have analyzed 681 hypothetical proteins using various functional prediction tools like CDART, Interproscan, SMART, Interpro, CATH and pfam. Among 682 total hypothetical proteins, we successfully annotated 436 proteins present in Neisseria meningitidis genome. It was observed that out of 436 proteins, 13 proteins are enzymes, 26 proteins are transporters, 11 are assembly proteins, 6 proteins involve in cell division, 70 proteins are binding proteins, 15 proteins are integral membrane proteins, 6 proteins are catalytic domains, 15 proteins are factors, 24 proteins regulators, 3 are structural proteins, 3 are ion channels, 42 are RNA proteins, 111 proteins sequences contain a domain of unknown function (DUF), remaining proteins cannot be functionally determined by any of the tools. These analyses suggest a possible role of hypothetical proteins in the survival, development and pathogenesis of the organism. Further we have identified 38 hypothetical proteins as virulence causing factors using VICMpred tool. Virulence causing proteins can serve as potent drug targets for the drug discovery process. The outcomes of this comprehensive study will be useful for better understanding of pathogenesis, drug resistance, adaptability to host, epidemic causes and drug discovery for treatment of the disease.

Biography:

Ali Morsali received his BS (1995) and MS (1999) in Coordination Polymers from Kharazmi (Tarbiat Moallem) and Zanjan Universities respectively, and his PhD (2003) in   Inorganic    Chemistry  from Tarbiat Modares University under the supervision of Professor Alireza Mahjoub. He is currently a faculty member in the Department of Chemistry of Tarbiat Modares Univers ity. He has been a Full Professor at the Tarbiat Modares University since 2011. His research interests include the solid-state reactivity involving coordination  polymers  and rational design and synthesis of metal-organic frameworks and their application in clean energy field and gas storage.        

Abstract:

In the domain of health, one important challenge is the efficient delivery of drugs in the body using   non-toxic   nanocarriers. Up  until now, two routes have been set up: The “organic route”, which uses either biocompatible polymers and the “ inorganic route ”, in which the hosts are inorganic porous solids, such as zeolites or mesoporous silicate materials. Most of t he existing carrier materials show poor drug loading and rapid release of the proportion of the drug that is simply adsorbed at the external surface of the nanocarrier. Herein, we introduce a third way: Porous inorganic-organic hybrid solids. Metal-organic frameworks (MOFs) are a new class of hybrid  crystalline  materials composed of organic linkers and metal nodes, with a diversity of structural characteristics and the nature of the pore surface. These materials have unquestionably enormous potential for many practical structure-related applications. This includes the more traditional areas of storage, separation or controlled release of gases, catalysis, sensing, and  d rug  delivery. I n this regards, we would like to introduce our azine/amide-functinalized pillar-layered TMU MOFs (TMU = Tarbiat Modares University) as potential drug delivery systems. For instance, the two 3D porous Zn(II)-based MOFs, containing azine-functionalized pores, [Zn₂(oba)₂(4-bpdb)]·(DMF)₂ (TMU-4) and [Zn₂(oba)₂(4-bpdh)]·(DMF)₂ (TMU-5) have showed acceptable capture of CO₂ with CO₂/N₂ selectivities of ~25 at 298 K. Moreover, a new MOF, TMU-30, based on isonicotinate N-oxide as an adsorptive site has been used for fast and highly efficient aqueous phase adsorption of Cr(VI) over a pH range of 2–9. Combination of high and regular porosity with the presence of functionalized-organic groups within  the TMUs can cumulate the advantages to achieve both a high drug loading and a controlled release.

Biography:

Ali Ramazani has completed his PhD under the supervision of Professor Issa Yavari in the Department of Chemistry at the Tarbiat Modares University (TMU), Iran. He currently works as a Full Professor in Chemistry at the University of Zanjan, Iran. His studies focused on Organic Synthesis and Nanochemistry. He has published more than 350 papers and is an Editorial Board Member of the international journal Nanochemistry Research. He has received several national and international awards, including the 2013 Khwarizmi International Award, Several Top-Cited Author Awards and Best-Paper Awards from leading ISI Journals, Best Researcher Awards and the Best Lecturer Awards at the University of Zanjan.

Abstract:

L-proline is known as the most favored catalyst in enamine-mediated reactions. This simple amino acid as a bifunctional catalyst is efficiently applied in variety of organic transformations. The simplicity of this small molecule contrasts with the complex structure of the natural enzymes, which are capable of promoting similar transformations. A secondary amine, functionality refers to its enhanced nucleophilicity over the other amino acids, which is the particular feature in nucleophilic catalysts. From the catalytic performance point of view, proline is termed “the simplest enzyme”, meanwhile it is a building block or catalytic center of some of natural enzymes. The surprising versatility and specificity of this simple natural amino acid against toxic organometallic catalysts convert it to a promising candidate for artificial enzyme designing. Magnetic functionalization of L-proline gives recoverability and reusability to this efficient organocatalyst. Herein, we report the synthesis and use of magnetite L-proline as an efficient and reusable  nano -biocatalyst in the coupling reaction of dimedone, malononitrile and aromatic  aldehydes to afford the corresponding benzo-[b]-pyran derivatives in aqueous media and in good yields. Pyran derivatives have great biological and pharmacological importance that is organized as a significant class of heterocyclic compounds. Despite the catalytic role of proline in chemical reactions, it has been known for several decades, but its significance in  biochemistry  and biogenesis has still remained uncovered. 

Biography:

Yui Umekawa is a PhD candidate in   biochemistry  ,  bioenergetics , molecular biology at The United  Graduate Sch ool of Agricultural Sciences, Iwate University, Japan.

Abstract:

Temperature is one of the most important requirements for all living    organisms   . To survive in severe environments in which temperature changes continuously,  some animals have gained the ability to maintain their temperature during the   evolutionary process  , called  homoeothemy , which is performed by a complex mechanism involving ther  mal receptors throughout the body and integration in the  hypothalamus  that controls shivering and non-shivering thermogenesis. Interestingly, flowers of some plants show a similar homeothermic b ehavior by inversely controlled respiration to temperature. To clarify the thermo regulatory mechanism in thermogenic plants, we investigated the temperature response of respiration in vivo using modified Arrhenius model using homeothermic spadices of skunk cabbage (Symplocarpus renifolius). Our results clearly showed that overall    thermodynamic    activation energy exhibits a negative value in the temperature range in which respiration control occurs. Our results suggest  that respiratory control in this plant is achieved by a   pre-equilibrium chemical   reaction in response to temperature. Moreover, citrate- driven respiration analysis using isolated   mitochondria   from  thermogenic spadices further suggests that chemically  endothermic  reactions, such as NADPH production catalyzed by mitoc  hondrial isocitrate dehydrogenase, are involved in our proposed pre-equilibrium reaction. A law of chemical equilibrium known as Le Châtelier's princip le may govern the homeothermic control in  skunk cabbage.

Biography:

Debora Roselita Karo Sekali has completed her Bachelor in Medical Science from Universitas Indonesia in 2016. She is currently pursuing her Honors Degree in Research of Reproductive Physiology of Women with prolonged labor in Monash University, Australia.

Abstract:

In Indonesia, approximately 20% of couples are infertile and 40% of the cases caused by male infertility. Impaired sperm motility (asthenozoospermia) is the leading cause of inability to conceive after regular unprotected sex in one year. Reactive Oxygen Species (ROS) is associated with male reproductive health. However, biochemical analysis on infertile males are rarely done in a clinical practice and WHO laboratory manual ranges of seminal fluid characteristic can be different in various races and ethnicities. Objective of this study is to compare malondialdehyde concentration as an indicator of ROS level in seminal plasma between normozoospermia and asthenozoospermia and to find the correlation between level of malondialdehyde and sperm motility among infertile males. Case control study analyzes 15 asthenozoospermic males and 20 normozoospermic males’ seminal plasma in a fertility clinic in Jakarta. Thiobarbituric acid assay is used to measure the concentration of malondialdehyde. Mann-Whitney test shows, there is no correlation between concentration of malondialdehyde and asthenozoospermia p value=0.194. However, average of malondialdehyde concentration in normozoospermia is lower than in asthenozoospermia. The future research with the same topic should be done by choosing fertile men as the control group and by taking minimal two samples for each subject since the population variances are unequal.

Biography:

Palina Vyhouskaya is a PhD student at the Jagiellonian University, Poland. She is a member of The Scientific Students Association of Laboratory  Diagnosticians , where she gains experience and practice connected with modern research methods used in Medicine. 

Abstract:

In oral cavity conditions, cariogenic bacteria   Streptococcus mutans   are characterized by altered   metabolism   compared to cells found in physiological flora. The metabolism of Streptococcus mutans is based on   glycolysis  , which also occurs in presence of oxygen (a phenomenon known as the Warburg effect). The low concentration of oxygen (<2%), i.e., hypoxia inside the biofilm, increases an expression of genes encoding glycolytic enzymes and i nhibits the oxidative  phosphorylation . Pyruvate kinase (PK) , one of the enzymes involved in glycolysis, is considered as an enzyme conditioning the rate of the whole process since it is activated by glucose-6-phosphate, a substrate of glycolysis. Pyruvate kinase  from  S. mutans ATCC and 40 clinical strains was purified, precipitated and estimated  fluorimetrically . Here we revealed the activity, and regulation of PK in mixed bacterial biofilm species and discuss how these properties enable regulation of PK for cariogenic biofilm proliferation and caries pro gression consequently. Clinical strains were isolated from children with caries. Mixed biofilm assay was carried out according to Current Protocols in Microbiology.PK activity was higher  (1.65 mU/mg of protein) in the mixed cariogenic biofilm species compared to the single and mixed physiological biofilm types (1.15 mU/mg of protein vs. 1.33 mU/mg of protein).It was demonstrated that the pyruvate kinase activity is increased in mixed cariogenic biofilm species. Streptococcus mutans are more resistant to glycolytic enzyme inactivation occurring in mixed cariogenic biofilm species (including Streptococcus sobrinus, Lactobacillus acidophilus, and Actinomyces viscosus) compared to mixed physiological biofilm types. Inhibition  of glycolytic enzymes might be an essential step in the reduction of mixed cario genic biofilm species which could be a useful tool in caries prophylaxis.

Biography:

Naureen Fatima has completed her studies from the Aligarh Muslim University (AMU), India. She has completed her graduation in 2009 and Post-graduation in 2011. In 2013, she has joined for her PhD at the Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, under the supervision of Dr. Shagufta Moin, Department of Biochemistry and co-supervision of Professor M Owais, Interdisciplinary Biotechnology Unit, Aligarh Muslim University. She has two research publications, one in Elsevier Journal, Appetite in 2013 as a second author and another in PLOS One in 2016 as a co-author. She has received the Best Poster Award in the 3^rd Annual Meeting of Indian Academy of Biomedical Sciences and Symposium on Modern Trends in Human Diseases in December, 2013 held at the Department of Biochemistry, J.N. Medical College, AMU, India.

Abstract:

Background: Curcumin, an active component of turmeric has caught tremendous attention as a potential therapeutics for diabetes because it is a relatively safe and inexpensive drug that reduces  glycemia  and hyperlipidemia in rodent models of diabetes. 

Aim: To study the effect of in-house synthesized curcumin nanoparticles (Cur NPs) in the treatment of type 2 diabetes in experimental Wistar rat model.

Method: The Streptozotocin (STZ)-induced experimental rat model of diabetes were used to evaluate the effect of in-house synthesized curcumin nanoparticles (Cur NPs) on glycemia, body weight, glycosylated  hemoglobin  (HbA1c), oxidative stress and inflammatory immunological markers. 

Results: In this study, we developed Aloe vera leaf extract (AVLE) mediated curcumin nano-formulation for highly effective diabetes therapy. Polyphenol (AVLE) mediated bio-functional, Cur NPs showed excellent dispersibility and outstanding stability in physiological environments. The data of biochemical and inflammatory  biomarkers  revealed the ameliorative effect of Cur NPs on STZ-induced diabetic experimental Wistar rat model. Interestingly, administration of Cur NPs for four weeks was able to prevent body weight loss, reduce the levels of glucose, hemoglobin (Hb), and HbA1C in blood and improve insulin sensitivity. 

Conclusions: The data of the present study clearly showed that the therapeutic efficacy of the AVLE synthesized Cur NPs were found better than that of free form of curcumin as well as with the AVLE alone. Cur NPs were also found to be effective in ameliorating the increased levels of fasting blood glucose, urine sugar, and urine volume in STZ-induced diabetic rats. Polyphenol mediated green synthesis of Cur NPs with effective and efficacious anti-diabetic potential may open new prospects for type 2 diabetes therapy.

Biography:

Zeba Farooqui is currently pursuing PhD at the Department of Biochemistry, Aligarh Muslim University (AMU), India. She is currently working on “protective effect of Nigella sativa and thymoquinone on cisplatin induced toxicity in rat kidney”. She has published a research article in an international journal. She has presented her work in several scientific meetings and conferences.

Abstract:

Nephrotoxicity is a severe complication in patients undergoing cisplatin (CP) chemotherapy. Thymoquinone (TQ), a monoterpene isolated from volatile oil of Nigella sativa seeds has been shown to exhibit strong antioxidant properties and protective effects against oxidative damage induced by several drugs and toxicants. The present study was undertaken to investigate whether TQ can prevent the CP-induced nephrotoxic effects or not. Rats were divided into four groups: Control, CP, TQ and CP+TQ. Rats in the groups CP+TQ and TQ were administered TQ (1.5 mg/kg bwt orally), prior to and simultaneously with and without, multiple doses of CP (3 mg/kg bwt, i.p) every fourth day for 20 days, respectively. CP nephrotoxicity was evident by increased serum creatinine (Scr) and blood urea nitrogen (BUN). CP treatment caused oxidant/antioxidant imbalances as reflected by increased  lipid  peroxidation (LPO), decreased enzymatic and non-enzymatic antioxidants. Furthermore, the activities of brush border membrane (BBM) marker enzymes like alkaline phosphatase (ALP), γ-glutamyl transferase (GGTase) and leucine aminopeptidase (LAP) were significantly declined in renal cortical and medullary homogenates and in isolated BBM vesicles (BBMV) in CP treated rats. Oral TQ administration, significantly attenuated CP induced increase in Scr and BUN and decrease in BBM enzymes activities. TQ administration also precluded CP induced alterations in the enzymatic and non-enzymatic antioxidant parameters. Histopathological observations showed extensive kidney damage in  CP treated animals and remarkably reduced renal injury in CP and TQ co-treated group. The results suggested that TQ alleviates CP induced nephrotoxicity and oxidative damage by strengthening antioxidant defense mechanism in the kidney.

Biography:

Eman T Mehanna is a Lecturer of Biochemistry and Molecular Biology at the Faculty of Pharmacy, Suez Canal University, Egypt. She has 6 internationally published papers.

Abstract:

The intestinal fatty acid binding protein (FABP-2) is expressed in enterocytes and binds with saturated and unsaturated long-chain fatty acids. FABP-2 Ala54Thr polymorphism was reported to have an influence on lipid metabolism. This study aimed to assess the relation of this polymorphism with peripheral atherosclerosis combined with type 2 diabetes mellitus in an Egyptian population. The study included 100 diabetic patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by PCR-RFLP. FABP-2 level was measured by ELISA technique. FBG, fasting serum insulin, HbA1c lipid profile, BMI, systolic and diastolic blood pressure were all determined. The Thr54 allele had higher frequency among the patients group (p=0.002). The heterozygote Ala54/Thr54 and the rare Thr54/Thr54 genotypes showed significant increase in BMI and FABP-2. Carriers of Thr54/Thr54 genotype had significantly decreased HDL-C. Carriers of Thr54/Thr54 genotype had significantly higher systolic and diastolic blood pressure than carriers of both Ala54/Ala54 and Ala54/Thr54 genotypes. FABP-2 level had positive correlation with BMI, systolic and diastolic blood pressure and negatively correlated with HDL-C. The Thr54 allele of FABP-2 Ala54Thr polymorphism was associated with increased incidence of peripheral atherosclerosis combined with type-2 diabetes mellitus in the studied population.

Biography:

Kavitha has double master’s in  Bioinformatics  and bachelor in Chemistry, currently doing phd in VIT,INDIA.She has be en actively engaged in research and done few international conferences and startup publishing work in various international publications.

Abstract:

    Norfloxacin     (fluroquinolones, noroxin) admission complex from claiming bigger dosage from claiming Norfloxacin might b ring about    Cholestatic      jaundice  .   Eventually Tom's perusing hindering alternately decreased bile stream in the liver. Those instruments from claiming Cholestatic jaundice might a chan ce to be comprehensively arranged under   hepatocellular  , the place an impedance for bile structuring happens also obstructive, the place impedance will bile stream happens after it is structured. Eclipta Prostrata may be recognized an essential liver herb clinched alongside Ayurveda. Those Eclipt a Prostrata need been extensively mulled over for its  hepatoprotective  movement. The hepatoprotective movement of the watery  extricate from claiming Eclipta Prostrata might have been investigated against Norfloxacin prompted Cholestatic jaundice clinched alongside rats. Level for serum ALT, AST also aggregate  bilirubin  might have been measured on normal, control (disease/toxicity induced), Furthermore watery extricate for Eclipta Prostrata dealt with rats. During the measurement from claiming 400 mg/kg, Norfloxacin prompted Cholestatic jaundice Previously, rats Concerning illustration showed Eventually Tom's perusing statistically  huge (p<0. 05) decline over serum ALT, AST Also downright bilirubin level contrasted with control bun ch. Oral pre-treatment about rats for the watery extricate for Eclipta Prostrata during the doses from claiming 150 mg/kg, 250 mg/kg and 350 mg/kg body weight former on Norfloxacin dosing in 400 mg/kg gotten huge distinction in the exercises for serum ALT, AST also downright bilirubin level of the test bunches. These present outcomes recommend that the watery extricate for Eclipta Prostrata might have a powerful hepatoprotective impact against Norfloxacin prompted Cholestatic jaundice rats.

Biography:

Naureen Fatima has completed her studies from the Aligarh Muslim University (AMU), India. She has completed her graduation in 2009 and Post-graduation in 2011. In 2013, she has joined for her PhD at the Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, under the supervision of Dr. Shagufta Moin, Department of Biochemistry and co-supervision of Professor M Owais, Interdisciplinary Biotechnology Unit, Aligarh Muslim University. She has two research publications, one in Elsevier Journal, Appetite in 2013 as a second author and another in PLOS One in 2016 as a co-author. She has received the Best Poster Award in the 3^rd Annual Meeting of Indian Academy of Biomedical Sciences and Symposium on Modern Trends in Human Diseases in December, 2013 held at the Department of Biochemistry, J.N. Medical College, AMU, India.

Abstract:

Curcumin, an active component of turmeric has caught tremendous attention as a potential therapeutics for diabetes because it is a relatively safe and inexpensive drug that reduces glycemia and hyperlipidemia in rodent models of diabetes.To study the effect of in-house synthesized curcumin nanoparticles (Cur NPs) in the treatment of type 2 diabetes in experimental Wistar rat model.The Streptozotocin (STZ)-induced experimental rat model of diabetes were used to evaluate the effect of in-house synthesized curcumin nanoparticles (Cur NPs) on glycemia, body weight, glycosylated hemoglobin (HbA1c), oxidative stress and inflammatory immunological markers.In this study, we developed Aloe vera leaf extract (AVLE) mediated curcumin nano-formulation for highly effective diabetes therapy. Polyphenol (AVLE) mediated bio-functional, Cur NPs showed excellent dispersibility and outstanding stability in physiological environments. The data of biochemical and inflammatory biomarkers revealed the ameliorative effect of Cur NPs on STZ-induced diabetic experimental Wistar rat model. Interestingly, administration of Cur NPs for four weeks was able to prevent body weight loss, reduce the levels of glucose, hemoglobin (Hb), and HbA1C in blood and improve insulin sensitivity.The data of the present study clearly showed that the therapeutic efficacy of the AVLE synthesized Cur NPs were found better than that of free form of curcumin as well as with the AVLE alone. Cur NPs were also found to be effective in ameliorating the increased levels of fasting blood glucose, urine sugar, and urine volume in STZ-induced diabetic rats. Polyphenol mediated green synthesis of Cur NPs with effective and efficacious anti-diabetic potential may open new prospects for type 2 diabetes therapy.

Biography:

Eman T Mehanna is a Lecturer of Biochemistry and Molecular Biology at the Faculty of Pharmacy, Suez Canal University, Egypt. She has 6 internationally published papers.

Abstract:

The intestinal fatty acid binding protein (FABP-2) is expressed in enterocytes and binds with saturated and unsaturated long-chain fatty acids. FABP-2 Ala54Thr polymorphism was reported to have an influence on lipid metabolism. This study aimed to assess the relation of this polymorphism with peripheral atherosclerosis combined with type 2 diabetes mellitus in an Egyptian population. The study included 100 diabetic patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by PCR-RFLP. FABP-2 level was measured by ELISA technique. FBG, fasting serum insulin, HbA1c lipid profile, BMI, systolic and diastolic blood pressure were all determined. The Thr54 allele had higher frequency among the patients group (p=0.002). The heterozygote Ala54/Thr54 and the rare Thr54/Thr54 genotypes showed significant increase in BMI and FABP-2. Carriers of Thr54/Thr54 genotype had significantly decreased HDL-C. Carriers of Thr54/Thr54 genotype had significantly higher systolic and diastolic blood pressure than carriers of both Ala54/Ala54 and Ala54/Thr54 genotypes. FABP-2 level had positive correlation with BMI, systolic and diastolic blood pressure and negatively correlated with HDL-C. The Thr54 allele of FABP-2 Ala54Thr polymorphism was associated with increased incidence of peripheral atherosclerosis combined with type-2 diabetes mellitus in the studied population.